Biological Chemistry
Staffs
Shin-ichi Hoshino, Ph.D., Professor
Tsuyoshi Udagawa, Ph.D., Associate Professor
Hiroto Inagaki, Ph.D., Research Associate
Tsuyoshi Udagawa, Ph.D., Associate Professor
Hiroto Inagaki, Ph.D., Research Associate
Research Project
- Mechanism of ハントカジノ 出金時間ecay in eukaryotes
- Posttranscriptional regulation of gene expression
- Mechanism of mRNA quality control (surveillance)
- Roles of eRF3 family G proteins in the regulation of ハントカジノ 出金時間ecay
Our laboratory conducts advanced research to gain a better understanding of the fundamental mechanisms of gene expression and RNA metabolism using genetic, molecular and biochemical approaches. We also focus upon the roles of RNA metabolism in cancer, apoptosis, aging, development and genetic diseases. The issues are being investigated in systems ranging from yeast to human cells.
Our efforts are currently devoted to understanding the molecular mechanism triggering ハントカジノ 出金時間ecay. In eukaryotes, poly(A)-tail shortening is the rate-limiting step in the degradation of most mRNAs, and two major ハントカジノ 出金時間eadenylase complexes, Caf1-Ccr4 and Pan2-Pan3, play central roles in this process, referred to as deadenylation. Previoiusly, we have demonstrated that translation termination factor eRF3 mediates deadenylation and decay of mRNA in a manner coupled to translation termination. Based on our recent work in yeast and mammal, we have proposed an initiation mechanism of ハントカジノ 出金時間egradation as described in Figure (3) (see below).
We have also demonstrated that tumor suppressor Tob mediates activation of ハントカジノ 出金時間eadenylase.
(1) How mRNA is degraded after completion of gene expression.
Our efforts are currently devoted to understanding the molecular mechanism triggering ハントカジノ 出金時間ecay. In eukaryotes, poly(A)-tail shortening is the rate-limiting step in the degradation of most mRNAs, and two major ハントカジノ 出金時間eadenylase complexes, Caf1-Ccr4 and Pan2-Pan3, play central roles in this process, referred to as deadenylation. Previoiusly, we have demonstrated that translation termination factor eRF3 mediates deadenylation and decay of mRNA in a manner coupled to translation termination. Based on our recent work in yeast and mammal, we have proposed an initiation mechanism of ハントカジノ 出金時間egradation as described in Figure (3) (see below).
We have also demonstrated that tumor suppressor Tob mediates activation of ハントカジノ 出金時間eadenylase.
(1) How mRNA is degraded after completion of gene expression.
After (or during) translation, mRNA is degraded. However, it has not been elucidated what triggers ハントカジノ 出金時間ecay.
(2) ハントカジノ 出金時間ecay pathway; deadenylation is the first and late-limiting step in degradation of most mRNAs.
During and after translation, poly(A) tails of mRNA is gradually shortened.
(3) Proposed model of ハントカジノ 出金時間eadenylation.
Translation termination complex eRF1-eRF3, Pan2-Pan3, ハントカジノ 出金時間af1-Ccr4 competitively interact with polyadenyate-binding protein PABPC1. In each complex, eRF3, Pan3, and Tob, respectively, mediate PABPC1 binding, and a combination of a PAM2 motif and a PABC domain is commonly utilized for their contacts. A translation-dependent exchange of eRF1-eRF3 for the deadenylase occurs on PABPC1. Consequently, PABPC1 binding leads to the activation of Pan2-Pan3 ハントカジノ 出金時間af1-Ccr4.
Translation termination complex eRF1-eRF3, Pan2-Pan3, ハントカジノ 出金時間af1-Ccr4 competitively interact with polyadenyate-binding protein PABPC1. In each complex, eRF3, Pan3, and Tob, respectively, mediate PABPC1 binding, and a combination of a PAM2 motif and a PABC domain is commonly utilized for their contacts. A translation-dependent exchange of eRF1-eRF3 for the deadenylase occurs on PABPC1. Consequently, PABPC1 binding leads to the activation of Pan2-Pan3 ハントカジノ 出金時間af1-Ccr4.
Contact Information
Department of Biological Chemistry,
Graduate School of Pharmaceutical Sciences, Nagoya City University,
3-1, Tanabe-dori, Mizuho-ku, Nagoya 467-8603, Japan
E-mail:hoshino@phar.nagoya-cu.ac.jp
TEL/FAX:+81-52-836-3427
Graduate School of Pharmaceutical Sciences, Nagoya City University,
3-1, Tanabe-dori, Mizuho-ku, Nagoya 467-8603, Japan
E-mail:hoshino@phar.nagoya-cu.ac.jp
TEL/FAX:+81-52-836-3427