Summary Text
A research group led by Kazunobu Sawamoto, a professor at Nagoya City University and National Institute for Physiological Sciences, and Chihiro Kurematsu, a student at Nagoya City University School of Medicine, has elucidated the mechanism that controls ハントカジノ ログイン new neurons in the adult brain. These findings are expected to be useful in the study of neurological diseases with abnormalities in microglia and synapses, such as autism spectrum disorders (ASDs).

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A research group led by Kazunobu Sawamoto, a professor at Institute of Brain Science, Nagoya City University Graduate School of Medical Sciences and National Institute for Physiological Sciences, and Chihiro Kurematsu, a fourth-year student at Nagoya City University School of Medicine, has elucidated the mechanism that controls ハントカジノ ログイン new neurons in the adult brain.

ハントカジノ ログインhe mammalian brain, neural stem cells exist even after birth, and new neurons are produced. As these new neurons mature, they form connections called synapses with existing neurons to create functional neural circuits. For the braハントカジノ ログインo develop and function normally, it is important to maintain an appropriate number of synapses, but the mechanism to regulate the number of synapses has not been fully understood. Cells called microglia, which exist near nerve cells, play an important role ハントカジノ ログインhis process. Microglia can "eat" (phagocytose) dead cells and are also known to digest extra synapses during development. However, it was unknown how new neurons ハントカジノ ログインhe adult brain eliminate extra synapses during maturation.
Sawamoto’s group focused on phosphatidylserine (PS), a molecule that usually resides inside the cell membrane, but is found on the external surface of dead cells or developing synapses, where it is recognized by microglia. First, the researchers used an electron microscope to examine the microglia in detail and observed that microglia actually engulf synapses. Next, they examined the localization of PS and found that PS is exposed outside the cell membrane at synapses ハントカジノ ログインhe adult mouse brain, especially at less active synapses.

“To study whether microglial PS detection is important for synaptic pruning and normal neuron maturation ハントカジノ ログインhe adult brain, we needed to see what happens when PS is masked in living adult mice,” Sawamoto said.

Finally, they generated genetically modified mice in which PS outside the cell membrane was masked by a protein, and observed microglia and synapses. As a result, they found that in these mice, microglia could not eat synapses properly, resulting in extra synapses left behind. Furthermore, neurons in these mice showed electrophysiological abnormalities. These results indicate that the ハントカジノ ログイン newborn neurons by microglia is PS-dependent in the adult brain, and that this mechanism is important for the correct maturation of newborn neurons.

Recent studies have shown that new neurons are also produced ハントカジノ ログインhe human brain during the neonatal period, and microglial synaptic pruning is believed to be important for postnatal brain development.

“We hope that investigating PS-dependent synaptic elimination in mouse models of brain diseases will lead to the development of new therapeutic strategies for human pathological conditions such as autism, where abnormalities in microglia and synaptic density have been observed,” said Kurematsu.

The full findings of the study are published ハントカジノ ログインhe Journal of Experimental Medicine.
Article title: Phosphatidylserine-dependent synaptic pruning by microglia ハントカジノ ログインhe maturation of adult-born neurons. DOI: 10.1084/jem.20202304

In addition to Kazunobu Sawamoto and Chihiro Kurematsu, co-authors of this research article include researchers from Nagoya City University, National Institute for Physiological Sciences, Hyogo College of Medicine, Aichi Developmental Disability Center, University of Medicine and Pharmacy of Ho Chi Minh City, Pham Ngoc Thach University of Medicine, National Institute of Neuroscience, Jichi Medical University, Tokushima Bunri University, Nagoya University, and Tokyo University of Pharmacy and Life Sciences.